Domain Binding Glutamate Elisa Kit Acid Rich 48t Size High
|Traditional ELISA Kit||Ready-to-Use ELISA KIT|
|Product name:||Human SH3 Domain-Binding Glutamic Acid-Rich-Like Protein 3(SH3L3)
SH3 domain-binding protein 1; SH3BP-1; P1725; SH3BGRL3
|Quality guarantee period:||for 12 months|
|Catalog number:||DL-SH3L3-Hu (traditional)||DLR-SH3L3-Hu (ready-to-use)|
- Competitive price.
- High sensitivity.
- High stability.
- 12 months shelf life.
- Pre-diluted Detection Reagent A and B
- Reduction in the number of steps when conducting the test
- All the reagents can be stored at 4℃
- Faster reaction compare to other brands
- 12 months shelf life
The kit is a sandwich enzyme immunoassay for the in vitro
quantitative measurement of SH3L3 in human serum, plasma, tissue
homogenates or other biological fluids.
|Composition||Traditional ELISA Kit||Ready-to-Use ELISA KIT||Conform|
|Pre-coated, ready to use 96-well strip plate 1||Pre-coated, ready to use 96-well strip plate 1|
|Plate sealer for 96 wells 2||Plate sealer for 96 wells 2|
|Standard 2||Standard 2|
|Diluents buffer 1×45mL||Standard Diluent 1×20mL|
|Detection Reagent A 1×120L||Detection Solution A 1×12mL|
|Detection Reagent B 1×120L||Detection Solution B 1×12mL|
|TMB Substrate 1×9mL||TMB Substrate 1×9mL|
|Stop Solution 1×6mL||Stop Solution 1×6mL|
|Wash Buffer (30concentrate) 1×20mL||Wash Buffer (30concentrate) 1×20mL|
|Instruction manual 1||Instruction manual 1|
The microtiter plate provided in this kit has been pre-coated with
an antibody specific to SH3L3. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated
antibody preparation specific to SH3L3. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and
After TMB substrate solution is added, only those wells that
contain SH3L3, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate
reaction is terminated by the addition of sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450nm10nm.
The concentration of SH3L3 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
Matrices listed below were spiked with certain level of recombinant
SH3L3 and the recovery rates were calculated by comparing the
measured value to the expected amount of SH3L3 in samples.
|Matrix||Recovery range (%)||Average(%)|
The linearity of the kit was assayed by testing samples spiked with
appropriate concentration of SH3L3 and their serial dilutions. The
results were demonstrated by the percentage of calculated
concentration to the expected.